Abstract:
The problem of hospital effluent discharges is increasingly important, as the drug-induced anthropization of hospital wastewater favors the emergence and dissemination in the environment of antibiotic-resistant microorganisms. The present study is part of the characterization of the multi-resistance to antibiotics of Staphylococcus spp. isolated from the effluents of a health establishment. For this purpose, 30 samples of wastewater were collected from the dialysis machines in the nephrology department of the Public Hospital Establishment of Telagh. Isolation is performed on Chapman medium and identification of the strains is performed by the API Staph gallery. The isolated strains were subjected to a standard antibiogram according to the recommendations of the Institute of Clinical and Laboratory Standards for different families of antibiotics (β-lactams, aminoglycosides, fluoroquinolones, and sulfonamides). Biochemical identification showed that our isolates were non-S. aureus strains, normally considered coagulase-negative, were found to be coagulase positive. Our results indicated that there is a passage of multidrug-resistant staphylococci in the environment, which requires periodic monitoring of staphylococci resistance to antimicrobials to control their spread.

Keywords: Hospital Effluent, Antibiotic Resistance, Staphylococci.